4/29/2023 0 Comments Microman pipette![]() We confirmed that the observable area is 261.4 and 13.9 times larger than that of a normal microscope and conventional view-expansive microscopic system, respectively. We evaluated the observable area of the system using this variable-focus device. Therefore, we propose the use of a variable-focus device to expand the visual field and thus circumvent the above-mentioned problems. The prototype of the view-expansive microscope has problems related to the variations in brightness and focal blur that accompany changes in the optical path length and amount of reflected light. In this study, we developed a microscopic image display apparatus for microinjections based on a view-expansive microscope. Additionally, microinjections are performed as the operator views a microscopic image on a display the position of the display requires the operator to maintain an awkward posture throughout the procedure. Microinjections are mainly performed based on visual information thus, the operator needs high-level skill because of the narrowness of the visual field. In recent years, the use of microinjections has increased in life science and biotechnology fields specific examples include artificial insemination and gene manipulation. ![]() For the purpose of spreading hamster ICSI, it is necessary to make further researches to improve manipulation techniques and to resolve experimental problems. Moreover, success rates of hamster ICSI remain very low. To our knowledge, there are no available reports on production of offspring from ICSI embryos in hamsters except our reports. The key points for hamster ICSI were in vitro manipulations under the light-controlled environment, injection of acrosome-less sperm heads into oocytes as soon as possible before spontaneous oocyte activation occurs, and determination of the optimal culture conditions. The authors summarized the history of hamster intracytoplasmic sperm injection (ICSI) and introduced key points for hamster ICSI, which were found in our previous studies on the production of embryos by ICSI and offspring by embryo transfer. Thus, biotechnological researches of hamster embryos should be performed with high-level skills of embryo manipulations. However, there are serious difficulties in doing in vitro manipulations of hamster embryos, because they are very sensitive to various environmental factors. Golden (Syrian) hamsters have many advantages for the study of reproductive biology and developmental biology, including a consistent estrous cycle, a stable superovulation response, and a short gestation period. We discuss lessons learned from the trials and possible improvements. We evaluate our system successfully both in a user study with untrained operators as well as a longer and more complex integrated mission. The head also carries a telepresence screen displaying a synthesized image of the operator with facial animation, which enables direct interaction with remote persons. Movement latency is hidden using spherical rendering. Finally, the robot features a 6D movable head with stereo cameras, which stream images to a VR HMD worn by the operator. The robot torso is mounted on a holonomic base, providing locomotion capability in typical indoor scenarios, controlled using a 3D rudder device. The remote human operator drives the arms and fingers through an exoskeleton-based operator station, which provides force feedback both at the wrist and for each finger. Our avatar robot features an anthropomorphic bimanual arm configuration with dexterous hands. We present such an avatar system, based on the key components of immersive 3D visualization and transparent force-feedback telemanipulation. Robotic avatars promise immersive teleoperation with human-like manipulation and communication capabilities. We verified that the proposed system improves the speed and accuracy of operating a pipette through two types of experiments with subjects. The operator can move the pipettes by 3D operation interface and freely change the viewpoint. In this system, an operator wearing a head-mounted display (HMD) and using 3D operation interfaces is immersed in a virtual micromanipulation space. The 3D operation interfaces are stationary pen-type or wearable glove-type interfaces. Our proposed system is composed of a real-time three-dimensional (3D) imaging microscope and 3D operation interfaces. Therefore, we propose an immersive micromanipulation system that requires no special skills for efficient and accurate micromanipulation. However, with the increasing demand for ART, the shortage of skilled embryologists has become a problem. ICSI is currently performed by specially skilled embryologists. The use of intracytoplasmic sperm injection (ICSI), an assisted reproductive technique (ART), is increasing widely.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |